Product Name :
VHR dual specificity phosphatase (human), (recombinant)
Sequence:
Purity:
≥95% (SDS-PAGE)
Molecular Weight:
20.4 kDa
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Use/Stability :
Description:
Dual specificity phosphatases (DUSPs) are members of the superfamily of protein tyrosine phosphatases. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. SDS-PAGE Analysis: Lane 1: MW Marker, Lane 2: 1 µg, Lane 3: 2 µg VHR dual-specificity phosphatase SDS-PAGE Analysis: Lane 1: MW Marker, Lane 2: 1 µg, Lane 3: 2 µg VHR dual-specificity phosphatase
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Solubility:
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Additional Information :
| Alternative Name Dual specificity protein phosphatase 3, DUSP3 | Application Notes Useful for the study of VHR dual phosphatase kinetics and substrate specificity, and to dephosphorylate pSer/Thr and pTyr phosphorylated protein substrates.1352226-88-0 Formula | Formulation Liquid.1186063-66-0 Purity In 50mM citrate, pH 6.PMID:25905203 0, containing 0.1M sodium chloride, 1mM EDTA and 1mM DTT. | MW 20.4 kDa | Purity ≥95% (SDS-PAGE) | Source Produced in E. coli. | Specific Activity 11U/mg. One unit will hydrolyze 1µmol p-nitrophenyl phosphate (pNPP) per minute at pH 6.0 and 30°C. Reaction conditions are 50mM citrate, pH 6.0, 0.1M NaCl, 1mM EDTA and 1mM DTT, 10mM pNPP substrate. An increase in signal of 3 to 4 fold can be achieved by addition of base to a pH of approximately 9.0 at the end of an assay with pNPP. | UniProt ID P51452
