Product Name :
PDI (human), (recombinant) (His-tag)
Sequence:
Purity:
≥95% (SDS-PAGE; Western blot)
Molecular Weight:
~58kDa
Solubility :
Appearance:
Use/Stability :
Description:
The mammalian protein disulfide-isomerase (PDI) family encompasses several highly divergent proteins involved in the processing and maturation of secretory proteins in the ER by catalyzing the rearrangement of disulfide bonds. PDI, an abundant protein of the ER (>400uM), contains a carboxy-terminal retention signal sequence, KDEL, similar to that of BiP and Grp94. The PDI proteins are characterized by the presence of one or more domains of ~95-110 amino acids related to the cytoplasmic protein thioredoxin. All but the PDI-D subfamily are composed entirely of repeats of such domains, with at least one domain containing – and one domain lacking – a redox-active Cys-X-X-Cys tetrapeptide.{{1809249-37-3} web|{1809249-37-3} Purity & Documentation|{1809249-37-3} Description|{1809249-37-3} custom synthesis} In addition to roles as redox catalysts and isomerases, PDI proteins perform such functions as peptide binding and cell adhesion, and may conduct chaperone activities1. Platelet surface thiols and disulfides play an important role in platelet responses. Catalytically active PDI resides on platelet surfaces where it mediates platelet aggregation and secretion by reducing disulfide bonds, thus exposing fibrinogen receptors in platelets. Enzymatic Activity: 1mg/ml insulin incubated with PDI in the presence of DTT – turbidity measured in 96-well microplate reader at 2 min intervals. SDS-PAGE analysis: Lane 1: MW marker, Lane 2: 0.5µg, Lane 3: 1µg, Lane 4: 2µg, Lane 5: 5µg PDI (human), (recombinant) (His-tag). Western Blot analysis: Lane 1: MWM, Lane 2: 50 ng PDI (human), (recombinant) (His-tag), probed with Prod. No. ADI-SPA-891. Enzymatic Activity: 1mg/ml insulin incubated with PDI in the presence of DTT – turbidity measured in 96-well microplate reader at 2 min intervals. SDS-PAGE analysis: Lane 1: MW marker, Lane 2: 0.5µg, Lane 3: 1µg, Lane 4: 2µg, Lane 5: 5µg PDI (human), (recombinant) (His-tag). Western Blot analysis: Lane 1: MWM, Lane 2: 50 ng PDI (human), (recombinant) (His-tag), probed with Prod.{{53-84-9} medchemexpress|{53-84-9} Protocol|{53-84-9} In stock|{53-84-9} manufacturer} No.PMID:29999807 ADI-SPA-891.
CAS :
Solubility:
Formula:
Additional Information :
| Activity Positive for disulfide isomerase activity, measured by promotion of insulin aggregation in the presence of DTT. | Alternative Name P4HB, protein disulfide isomerase | Application Notes Western blot control. | Formulation Liquid. In 50mM TRIS, pH 7.5, containing 150mM sodium chloride, 1mM DTT, 1mM EDTA, and 5% glycerol. | MW ~58kDa | Purity ≥95% (SDS-PAGE; Western blot) | Purity Detail Purified by multi-step chromatography. | Source Produced in E. coli. Human PDI is fused at the N-terminus to a His-tag. | Specific Activity >200 U/mg. 1 U increases the A650 of a 1mg/ml insulin solution by .01 OD/min at 25°C in the presence of DTT. | UniProt ID P07237
