Fungus DGGE 1 two 3 four five 6 7 8 9 ten 11 12 13 14 15 Bacillus DGGE 1 2 3 four 5 6 7 8 9 ten Alphaproteobacterium DGGE 1 2 three 4 five six 7 eight 9 ten 11 12 13 Pseudomonas DGGE 1 2 three four 5 6aSoil Gb four 2 0 0 0 0 0 four four four 0 two 0 four 2 Kw four 0 0 0 0 0 two 0 0 0 0 four 0 four 0 Go four 0 0 0 four 0 0 0 0 4 0 four 0 four 0 Gb four 0 0 0 0 0 0 4 0 four 0 four 0 4Closest GenBank match (organism, GenBank no.) Malassezia restricta, EU400587 Aspergillus penicillioides, GU017496 Cryptococcus pseudolongus, AB105353 Chaetomium globosum, JX501299 Arthopyreniaceae, FJ439584 Eurotium sp., AM901702 Ganoderma applanatum, JX501311 Cladosporinum cladosporioides, AJ300335 Davidiella sp., JX164064 Cryptococcus sp., JX164076 Trichosporonales, EF060720 Mortierella sp., JF439489 Cylindrocarpon olidum, GU198183 Ascomycete, AM410609 Rhizophydium sp., DQaidentity 98.7 99.6 one hundred 98.two 100 100 99.six one hundred 99.6 100.0 98.3 99.six 99.0 99.two 98.Kw four 4 0 0 0 four 4 0 4 0 4 4 4 4Go 4 0 4 4 four 0 0 0 4 0 4 four 0 4Bradyrhizobium pachyrhizi, NR_043037 Sphingomonas insulae, NR_044187 Staphylococcus epidermidis, NR_036904 Staphylococcus epidermidis, NR_036904 Micrococcus endophyticus, NR_044365 Bacillus megaterium, NR_043401 Micrococcus luteus, NR_037113 Propionibacterium acnes, NR_040847 Methylobacterium rhodesianum, NR_041028 Streptococcus thermophilus, NR_97.9 99.4 100 99.6 98.6 99.7 99.two 100 97.20 1 4 4 three 4 4 4 23 1 four 4 3 4 four 4 ten three four four four four 4 four 30 0 0 0 0 0 four 4 00 0 0 0 0 0 4 four 00 0 0 0 0 0 4 4 0Solirubrobacter soli, NR_041365 Janthinobacterium lividum, NR_026365 Rhizobium phaseoli, NR_044112 Pedomicrobium australicum, NR_026337 Ochrobactrum anthropi, NR_074243 Hyphomonadaceae, NR_041967 Nitrospira moscoviensis, NR_029287 Rhodobiaceae, NR_042626 Devosia chinhatensis, NR_044214 Kaistia soli, NR_044302 Magnetospirillum gryphiswaldense, NR_027605 Bosea eneae, NR_028798 Rhodobacter blasticus, NR_99.8 99.8 99.eight 96.0 99.five 91.0 96.three 92.eight 96.six 96.0 96.3 95.5 96.two 1 4 1 four three two two 0 0 1 43 0 4 3 3 2 three 2 3 2 three 41 3 four 3 2 3 0 two two 3 1 43 0 0 four two 4 0 0 four 0 0 03 0 0 4 4 four 0 0 four 0 0 00 0 0 four 0 four 0 0 four 0 0 0Pseudomonas asplenii, NR_040802 Pseudomonas tuomuerensis, NR_043990 Pseudomonas koreensis, NR_025228 Pseudomonas jessenii, NR_024918 Pseudomonas jessenii, NR_024918 Pseudomonas koreensis, NR_025228 Pseudomonas taetrolens, NR_99.Carmustine five 99.Mebendazole 1 100 99.three 99.1 99.eight 98.0 2 3 1 1 33 3 0 three 1 00 2 0 three three 10 4 0 0 0 00 4 0 4 0 00 four 0 3 4 0Details for the BLASTN results and taxonomy are provided inside the supplemental material. (T), variety strain.largely below the detection limit in the soil fungal communities. Some bands had been widespread on both nematodes and soil samples within the 3 soils, including bands 1, 12, and 14, which corresponded to Malassezia restricta, Mortierella sp., and Ascomycete sp.PMID:23833812 , respec-tively (Table 2). Eight of the ITS sorts linked to J2 were soil sort specific, four of which had been only detected on J2 (Table two, bands three, four, six, and 13), though the other 4 had been obtained from each J2 and soil samples (Table 2, bands five, 7, 8, and ten). Theaem.asm.orgApplied and Environmental MicrobiologyMicrobes Attached to Root Knot Nematodes in SoilFIG two DGGE profiles of bacterial 16S rRNA genes amplified from DNA of M. hapla J2from three arable soils and from total soil DNA. A, B, C, and D refer to replicate soil baiting assays for every soil.sequences of these bands exhibited 98 to one hundred similarity to known sequences of fungal species in GenBank (Table 2). Furthermore, two of your attached ITS varieties seemed to become precise for J2 samples in two with the 3 soils (Table two, bands 2 an.
M2 ion-channel m2ion-channel.com
Just another WordPress site
