Min in salivary and gastric fractions. Moreover, its isomer was

Min in salivary and gastric fractions. Moreover, its isomer was registered at Rt = 70.5 min. Furthermore, in all gastrointestinal fractions, aromadendrin was detected and assigned according to the principle ion m/z 287 [M – H]- at Rt = 47.2 min [27]. It really is supposed that within the limited air conditions, the aglycone of kaempferol is lowered to aromadendrin. It really is worth noting that the peak of aromadendrin was even more intensive in FS(+E) fraction than in FS(-E), which possibly suggests that enzymatic hydrolysis of aromadendrin hexoside requires spot inside the bacterial atmosphere. The principle ion of aromadendrin hexosides was m/z 449 [M – H]- within the adverse ESI mode registered at Rt = 25.6 min and Rt = 29.7 min. They had been discovered in all fractions from the gastrointestinal pathway. Another flavonol identified inside the gastrointestinal fractions immediately after digestion of CM extract was quercetin and its derivatives. In the gastric fractions, a compound characterized by the primary ion m/z 477 [M H]- was noted at Rt = 45.1 min. Its fragmentary ion was m/z 301 in adverse ESI mode. Thus, the compound was tentatively identified as quercetin glucuronide [26,28]. In addition, inside the salivary, gastric, and intestinal fractions dihydroquercetin (syn. Taxifolin) was assigned. In negative ESI mode, its major ion was m/z 303 [M – H]- at Rt = 43.1 min, whereas the fragmentary ion was m/z 163 [29]. In all gastrointestinal fractions obtained in vitro, the signals corresponding to tannins have been detected. The compounds, of which big ions in the MS spectrum had been m/z 513 [M – H]- (Rt = 14.0 min and Rt = 16.4 min), were located in the damaging ESI mode (Figure 3B).Ginsenoside Rg1 References Their MS2 fragmentation pattern showed signals at m/z 495, 343, 271, 241, and 169.MNS In Vivo The compounds were assigned to gallic acid derivatives.PMID:23551549 The isomers with the compound, which showed a signal at m/z 593 (Rt = 6.five min and Rt = 16.4 min) inside the adverse mode (CM_S, CM_I, and CM_FS), supplied fragmentary ions which include 547, 513, 503, and 333. Equivalent fragmentary ions have been identified inside the case of a compound described by m/z 603 in the negative ESI mode. In some analytes, the MS2 fragmentation pattern of the ion showed a signal at m/z 337 in the adverse ESI mode. Thus, we suppose that it could possibly be a derivative of p-coumaroylquinic acid. The compounds identified as hydrolyzable tannins were identified at Rt = 14.0 min and Rt = 18.6 min (CM_G). The key ion in their MS spectrum was m/z 783 [M – H]- . Its important MS2 fragmentation pattern showed a signal at m/z 483 characteristic for digalloyl glucose. Thus, they have been tentatively assigned to cornusiin A [30]. The ion supplying a signal at m/z 787 (FS_(-E) (Rt = 42.five min), also as fragmentary ions at m/z 635 (tri-O-galloyl-D-glucose), 617, 465, and 303, was tentatively assigned to tetra-O-galloyl–D-glucose in CM_G [30]. Benzoic acid, of which the primary ion is [M – H]- m/z 121, was detected in the adverse ESI mode at Rt = 18.7 min in analytes from salivary to FS fractions. Its quantity seems to enhance substantially in specific inside the analytes incubated with FS. In addition, an ion [M – H]- m/z 299, discovered in all gastrointestinal fractions except for gastric one, was tentatively assigned to hexoside of hydroxybenzoic acid [M – H]- m/z 137.ESI mode at Rt = 18.7 min in analytes from salivary to FS fractions. Its quantity seem improve substantially in unique within the analytes incubated with FS. Additionally, an [M – H]- m/z 299, identified in all gastrointestinal fractions except for gastri.