(1113 significantly upregulated and 956 significan downregulated), 334 DEGs (155 significantly upregulated and 179 drastically

(1113 significantly upregulated and 956 significan downregulated), 334 DEGs (155 significantly upregulated and 179 drastically dow regulated), 321 DEGs (131 considerably upregulated and 190 significantly downreguAnimals 2021, 11, x FOR PEER REVIEW6 ofAnimals 2021, 11,ed), and 1423 DEGs (582 considerably upregulated and 841 considerably downregulated), respectively. By analyzing a Venn diagram (Figure 4G), it really is evident that there were 63, 329, 1678, 137, 119, and 970 exceptional differentially expressed genes within the duodenum, PI4KIIIα Purity & Documentation jejunum, ileum, cecum, colon, and rectum, respectively.six ofA.B.C.D.E.F.G.H.Figure three. Differential gene volcano map, differential gene Wayne map, and differential gene quantity histogram. (A ) The Vocalo diagram analysis of DEGs TBK1 Storage & Stability inside the duodenum, jejunum, ileum, cecum, colon, and rectum in turn. Red indicates substantially upregulated DEG, and blue indicates considerably downregulated DEG (|log2(foldchange)| 1 and p-value 0.05). (G) Differential gene Venn diagram; diverse colors indicate unique comparison combinations. (H) The histogram on the quantity of differentially expressed genes is shown in blue and gray. The number around the column represents the amount of differentially expressed genes. S_Z: the duodenum of healthier rabbits, S_B: diarrhea in the duodenum of rabbits, H_Z: wholesome rabbit ileum, H_B: diarrheal rabbit ileum, K_Z: healthy rabbit jejunum, K_B: rabbits with diarrheal jejunum, M_Z: wholesome cecum of rabbits, M_B: rabbits with diarrheal cecum, J_Z: healthy rabbit colon, J_B: colon of rabbits with diarrhea, Z_Z: healthy rabbit rectum, Z_B: rectum of rabbits with diarrhea.36.Yunpeng, C.; Shihua, P.; Jianjun, Z.; Weijie, Z.; Huijuan, N.; Zhicai, Q.; Jingwen, W.; Daleng, S.; Zengji, Y. RAPD Evaluation of Genetic Relationships amongst All-natural Populations of Hybrid Taxodium Mucronatum Tenore. Fu Dan Xue Bao Zi Ran Ke Xue Ban J. Fudan Univ. Nat. Sci. 2002, 41, 64145.Animals 2021, 11,7 ofBiosensors 2021, 11, x FOR PEER REVIEW13 ofFigure 4. Cont.Animals 2021, 11,eight ofFigure four. GO enrichment analysis of DEGs in distinct comparison groups. S_Z vs. S_B (A), K_Z vs. K_B (B), H_Z vs. H_B (C), M_Z vs. M_B (D), J_Z vs. J_B (E), and Z_Z vs. Z_B (F). GO terms are around the x-axis. The enrichment ratio of genes is shown as GO terms for BP, CC, and MF. S_Z: the duodenum of healthy rabbits, S_B: diarrhea in the duodenum of rabbits, H_Z: healthy rabbit ileum, H_B: rabbit with diarrheal ileum, K_Z: wholesome rabbit jejunum, K_B: rabbits with diarrheal jejunum, M_Z: healthful cecum of rabbits, M_B: rabbits with diarrheal cecum, J_Z: healthier rabbit colon, J_B: colon of rabbits with diarrhea, Z_Z: healthy rabbit rectum, Z_B: rectum of rabbits with diarrhea.3.5. Enrichment Analysis of GO and KEGG Pathway Compared together with the general description on the properties of genes or transcripts with functional annotation, the lowest amount of gene function and KEGG pathway may be annotated by enrichment analyses. In addition, enrichment evaluation gives one of the most detailed information regarding gene function and KEGG pathway, which will help us to screen exceptional insights on diarrhea response in rabbits fed a no-antibiotic diet program. Moreover, GO terms and KEGG pathways that satisfy the corrected p-value of 0.05 had been considered considerably enriched. To classify and characterize DEG functions and pathways, we performed a gene ontology (GO) classification and functional annotation of molecular biological function, cellular components, and biological approach (F