ntributions NM, GJD, HSO, and JGB developed and planned the research. MH ready fungal cultures. CB and SS prepared activitybased probes applied within this study. NM collected secretome samples and performed activitybased protein profil ing experiments. NM collected and analysed proteomic data. DN performed bioinformatic evaluation. NM and MS prepared P. pastoris strains, produced and purified recombinant enzymes, and performed activity assays. NM wrote the manuscript with input from all the authors. All authors study and approved the final manuscript. Funding The authors thank the Natural Sciences and Engineering Research Council of Canada (PostDoctoral Fellowship to NGSM), the Royal Society (Ken Murray Analysis Professorship to GJD), the Biotechnology and BRD9 Storage & Stability Biological Sciences Study Council (BBSRC) (grant BB/R001162/1 to GJD), the French National Investigation Agency (ANR13BIME0002 to JGB), the Netherlands Organization for Scientific Study (NWO Top rated grant 2018714.018.002 to HSO), along with the European Analysis Council (ERC2011AdG290836 “Chembiosphing” to HSO, ERC2020SyG951231 “Carbocentre” to GJD and HSO). Proteomics information were collected at the York Centre of Excellence in Mass Spectrometry, which was made due to a significant capital investment through Science City York, sup ported by Yorkshire Forward with funds from the Northern Way Initiative, and subsequent help from EPSRC (EP/K039660/1; EP/M028127/1). Availability of information and supplies Pichia pastoris strains and samples of recombinant proteins may well be out there from Gideon Davies ([email protected]). Samples of ABPCel, ABPXyl, and ABPGlc may be readily available from Herman Overkleeft (h.s.overkleeft@lic. leidenuniv.nl). Basidiomycete fungi are obtainable in the fungal culture collection on the International Centre of Microbial Sources (CIRMCF) in the French National Institute for Agricultural research (INRA; Marseille, France). Genome sequences for each in the fungi made use of in this study are accessible from Mycocosm (mycocosm.jgi.doe.gov/mycocosm/home) (DOE Joint Genome Institute, Walnut Creek, California). Other datasets utilised and/or ana lysed in the course of the present study are obtainable in the corresponding author on reasonable request.Author details 1 York Structural Biology Laboratory, Division of Chemistry, The University of York, Heslington YO10 5DD, York, UK. 2 Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA Leiden, The Netherlands. 3 UMR1163 Bio diversitet Biotechnologie Fongiques, Facultdes Sciences de Luminy, INRAE, Aix GSK-3α Formulation Marseille Univ, 13288 Marseille, France. four Polytech Marseille, Aix Marseille Univ, 13288 Marseille, France. Received: 8 October 2021 Accepted: six JanuaryDeclarationsEthics approval and consent to participate Not applicable. Consent for publication Not applicable. Competing interests The authors declare no competing interests.References 1. Scheller HV, Ulvskov P. Hemicelluloses. Annu Rev Plant Biol. 2010;two(61):2639. two. Luis AS, Briggs J, Zhang X, Farnell B, Ndeh D, Labourel A, et al. Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018;3(two):210. 3. Celiska E, Nicaud JM, Bialas W. Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide resources: overview on starch, cellulose, xylan, and inulin. Appl Microbiol Biotechnol. 2021;105(three):9759. 4. Schlembach I, Hosseinpour Tehrani H, Blank LM, B hs J, Wierckx N, Regestein L, et al. Consolidate
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