ntributions NM, GJD, HSO, and JGB developed and planned the study. MH ready fungal cultures.

ntributions NM, GJD, HSO, and JGB developed and planned the study. MH ready fungal cultures. CB and SS ready activitybased probes applied in this study. NM collected secretome samples and performed activitybased protein profil ing experiments. NM collected and analysed proteomic data. DN performed bioinformatic analysis. NM and MS prepared P. pastoris strains, created and purified recombinant enzymes, and performed activity assays. NM wrote the manuscript with input from all the authors. All authors read and approved the final manuscript. Funding The authors thank the Organic Sciences and engineering Research Council of Canada (PostDoctoral Fellowship to NGSM), the Royal Society (Ken Murray Research HDAC9 Purity & Documentation Professorship to GJD), the Biotechnology and Biological Sciences Study Council (BBSRC) (grant BB/R001162/1 to GJD), the French National Study Agency (ANR13BIME0002 to JGB), the Netherlands Organization for Scientific Study (NWO Leading grant 2018714.018.002 to HSO), along with the European Research Council (ERC2011AdG290836 “Chembiosphing” to HSO, ERC2020SyG951231 “Carbocentre” to GJD and HSO). Proteomics information have been collected at the York Centre of Excellence in Mass Spectrometry, which was produced due to a major capital investment through Science City York, sup ported by Yorkshire Forward with funds in the Northern Way Initiative, and subsequent help from EPSRC (EP/K039660/1; EP/M028127/1). Availability of data and supplies Pichia pastoris strains and samples of recombinant proteins may be available from Gideon Davies ([email protected]). Samples of ABPCel, ABPXyl, and ABPGlc may possibly be readily available from Herman Overkleeft (h.s.overkleeft@lic. leidenuniv.nl). Basidiomycete fungi are available in the fungal culture collection of your International Centre of Microbial Sources (CIRMCF) at the French National Institute for Agricultural study (INRA; Marseille, France). Genome sequences for each and every in the fungi employed in this study are offered from Mycocosm (mycocosm.jgi.doe.gov/mycocosm/home) (DOE Joint Genome Institute, Walnut Creek, California). Other datasets utilised and/or ana lysed through the present study are out there in the corresponding author on affordable request.Author details 1 York Structural Biology Laboratory, Department of Chemistry, The University of York, Heslington YO10 5DD, York, UK. 2 Leiden Institute of Chemistry, Leiden University, ACAT1 list Einsteinweg 55, 2300 RA Leiden, The Netherlands. three UMR1163 Bio diversitet Biotechnologie Fongiques, Facultdes Sciences de Luminy, INRAE, Aix Marseille Univ, 13288 Marseille, France. four Polytech Marseille, Aix Marseille Univ, 13288 Marseille, France. Received: 8 October 2021 Accepted: six JanuaryDeclarationsEthics approval and consent to participate Not applicable. Consent for publication Not applicable. Competing interests The authors declare no competing interests.References 1. Scheller HV, Ulvskov P. Hemicelluloses. Annu Rev Plant Biol. 2010;2(61):2639. two. Luis AS, Briggs J, Zhang X, Farnell B, Ndeh D, Labourel A, et al. Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018;3(two):210. three. Celiska E, Nicaud JM, Bialas W. Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide resources: evaluation on starch, cellulose, xylan, and inulin. Appl Microbiol Biotechnol. 2021;105(3):9759. four. Schlembach I, Hosseinpour Tehrani H, Blank LM, B hs J, Wierckx N, Regestein L, et al. Consolidate