Of photosynthetic prices (f), biomass (g), C content material (h) and C:N ratio (i) amongst

Of photosynthetic prices (f), biomass (g), C content material (h) and C:N ratio (i) amongst NJ6, NJ6-sd1 and NJ6-sd1-OsGRF4ngr2 plants. Data shown as imply s.e.m. (n = 30). Various letters denote substantial variations (P 0.05, Duncan’s several variety test). j, Relative shoot abundances of mRNAs transcribed from cell-cycle regulatory genes in NJ6, NJ6-sd1 and NJ6-sd1-OsGFR4ngr2 plants. Transcription relative towards the level in NJ6-sd1 plants (set to a single). Data shown as imply s.e.m. (n = three). Diverse letters denote significant variations (P 0.05, Duncan’s several variety test). k, ChIP-PCR assays. Diagram depicts the OscycA1.1 and Oscdc2Os-3 promoters and regions (GCGGcontaining fragment marked with ) utilized for ChIP-PCR. Data shown as mean s.e.m. (n = three). Diverse letters denote important differences (P 0.05, Duncan’s numerous variety test).l, Transactivation assays in the OscycA1.1 and Oscdc2Os-3 promoters. Information shown as mean s.e.m. (n = 12). Distinct letters denote important differences (P 0.05, Duncan’s a number of range test).Europe PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsExtended Data Figure eight. Natural allelic variation at OsGRF4 is associated with variation in plant and grain morphology and grain yield performance.a, DNA polymorphisms in the promoter area of OsGRF4. Green-shaded regions indicate the 3 one of a kind SNP variations related with phenotypic variation in NM73 and RD23. bf, Boxplots for plant height (b), grain length (c), grain width (d), the amount of grains perNature. Author manuscript; offered in PMC 2019 February 15.Li et al.Pagepanicle (e), and grain yield functionality (f) of rice varieties carrying different OsGRF4 promoter haplotypes (Hap.; A, B or C). All data from plants grown in standard paddy-field fertilization conditions22. Data shown as imply s.e.m. (Hap. A, n = 74; Hap. B, n = 28; Hap. C, n = 123). The violin map was constructed in R. Diverse letters above columns indicate statistically substantial variations involving groups (Tukey’s honestly substantial distinction (HSD) test, P 0.05; panels b-f).Europe PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsExtended Information Figure 9. Agronomic traits displayed by 9311 and 9311-OsGRF4ngr2 plants grown at varying N fertilisation levels.a, Flag leaf width. Data shown as mean s.e.m. (n = 30). A TCID manufacturer two-sided Student’s t-test was made use of to generate the P values. b, Culm width of your uppermost internode. Information shown as mean s.e.m. (n = 30). A two-sided Student’s t-test was utilised to produce the P values. c, 15NH + uptake. d, 15NO – uptake. Data shown as imply s.e.m. (n = 30). A two-sided 4 three Student’s t-test was made use of to produce the P values. Rice root 15NH4+ (c) and 15NO3- (d) uptake prices of 4-week old plants grown in varying N supply (0.15N, 0.1875 mM NH4NO3; 0.3N, 0.375 mM NH4NO3; 0.6N, 0.75 mM NH4NO3; 1N, 1.25 mM NH4NO3). e, The amount of grains per panicle. Data shown as mean s.e.m. (n = 30). A two-sided Student’s t-test was applied to generate the P values. f, 1,000-grain weight. Information shown as mean s.e.m. (n = 30). A two-sided Student’s t-test was employed to produce the P values. g, Harvest index. Information shown as mean s.e.m. (n = 30). A two-sided Student’s t-test was utilised to produce theNature. Author manuscript; offered in PMC 2019 February 15.Li et al.PageP values. h, Dry biomass per plant. Data shown as imply s.e.m. (n = 30). A two-sided Student’s t-test was employed to produce the P values.Europe PMC Funders Author Manuscripts Europe P.