Present study. The characteristics of the study subjects are given inPresent study. The characteristics of

Present study. The characteristics of the study subjects are given in
Present study. The characteristics of the study subjects are given in Table 1. Although the Trichostatin A dose patients with IPF and EP had significantly lower vital capacity predicted ( VC) than the healthy subjects, and the patients with IPF had significantly lower total lung capacity predicted ( TLC), functional residual capacity predicted ( FRC), residual volume (RV), RV predicted ( RV), and diffusion lung carbon monoxide predicted ( DLCO) than the patients with EP, there was no significant difference in other values of lung function among three groups. Although eosinophil counts in BALf were not so high in some patients with EP in this study, eosinophil infiltration into the alveolar septa was observed in the lung tissues from all EP patients (Additional file 1, Figure S1).Exhaled NO levels in the study subjectsThe FENO levels in the EP group (35.0 ?5.2 ppb) were significantly higher than in the healthy subject group (17.8 ?2.2 ppb, p < 0.01) and the IPF group (20.8 ?1.8 ppb, p < 0.01, Figure 1A). Because eosinophilic inflammation occurs in the lung parenchyma in EP, weFurukawa et al. Respiratory Research 2011, 12:81 http://respiratory-research.com/content/12/1/Page 4 ofTable 1 Characteristics of the study subjectsHS Number (M/F) Age (yrs ) Smoking status (never-/ex-/ current smoker) VC (L) VC ( ) FEV1.0 (L) FEV1.0 ( ) TLC (L) TLC ( ) FRC (L) FRC ( ) RV (L) RV ( ) DLCO ( ) DLCO/VA ( ) 10(4/6) 60.9 ?4.5 (6/4/0) IPF 13(12/1) 69.5 ?1.9 (1/12/0) EP 13(7/6) 63.2 ?3.6 (8/5/0)3.20 ?0.18 2.94 ?0.23 2.57 ?0.25 108 ?3.6 80.5 ?2.6 N.D. N.D N.D. N.D N.D. N.D. N.D. N.D. 87.0 ?5.8* 86.9 ?7.0* 80.8 ?1.6 73.7 ?6.0 82.4 ?2.5 96.5 ?7.1 2.54 ?0.16 2.40 ?0.16 2.08 ?0.18 4.04 ?0.32 4.61 ?0.45 2.40 ?0.14 2.86 ?0.24 74.9 ?6.5 97.8 ?6.9 1.34 ?0.13 66.4 ?9.9 66.7 ?5.4 73.6 ?5.4 1.90 ?0.19 115 ?14 91.5 ?11 86.7 ?6.observed in the patients with EP than in the healthy subject and IPF patient (Figure 3A-C, Additional file 3, Table S2). There were significant positive correlations between the proportion of 3-NT positive cells and the FE NO levels (r = 0.890, p < 0.01, Figure 3D), JawNO levels (r = 0.790, p < 0.05), or the Calv levels (r = 0.924, p < 0.01, Figure 3E). The proportion of iNOS positive cells was significantly correlated with that of 3-NT positive cells (r = 0.919, p < 0.01, Figure 4).Correlation between the exhaled NO levels and lung function or inflammatory markersHS = healthy subject; IPF = idiopathic pulmonary fibrosis; EP = eosinophilic pneumonia; VC = vital capacity; VC = VC predicted; FEV1.0 = forced expiratory volume in one second; TLC = total lung capacity; TLC = TLC predicted; FRC = functional residual capacity; FRC = FRC predicted; RV = residual volume; RV = RV predicted; DLCO = diffusion lung carbon monoxide predicted; DLCO/VA = DLCO/alveolar volume predicted; N.D. = not done. *p < 0.05 compared with the values of HS group; p < 0.05 compared with the values PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26577270 of IPF group.We examined the correlation between the exhaled NO levels and the values of lung function and systemic inflammatory markers in the patients with EP before systemic steroid treatment (Table 2). There were significant correlations between the Calv levels and VC (r = -0.670, p < 0.05), VC (r = -0.645, p < 0.05), forced expiratory volume in one second (FEV1.0) (r = -0.662, p < 0.05) or peripheral blood eosinophil counts (r = 0.658, p < 0.05).Analysis of cytokine and chemokine profile in EBCspeculated that the Calv levels in the EP group would be elevated com.