, ages if reported) 103 WSW, including 21 monogamous couples 91 women Setting Number BV

, ages if reported) 103 WSW, including 21 monogamous couples 91 women Setting MG-132 dose Number BV positive/ Total ( ) Diagnostic method(s) ASBerger et al,1995 [13] McCaffrey et al, 1999 [16]a Marrazzo et al,2002 [15]USACross-sectionalGynaecology practice, GSK-AHAB web Community clinic Specialist lesbian genitourinary clinic Community recruitmentPrevalence 29 / 101 (28.7) ?2 ungradeable Prevalence 47 / 91 (51.6)UKCross-sectionalHay-Ison criteria NSUSACross-sectional326 WSW, including 58 monogamous couples, age 16 years 708 WSW, age 16?3 years 171 WSW, 189 heterosexual women, age 16?0 years 335 WSW, age 16?0 years jir.2010.0097 237 WSW, age 16 years 335 WSW, age 16?5 years 335 WSW, age 16?5 years 89 WSW, age 16?0 years 193 women, age 17?21 yearsc 146 symptomatic womencPrevalence 81 / 326 (25)Bailey et al, 2004 [31] Evans et al, 2007[14]bbUKCross-sectionalNew patients at a lesbian/ bisexual sexual health clinic Community recruitment Community recruitment Community recruitment Community recruitment Community recruitment Community recruitment University recruitment Sexual health clinic STD Clinic Community recruitment, STI clinic, GP Clinic Community recruitment, STI clinic, GP clinicPrevalence 222 / 708 (31.4)ASUKCross-sectionalPrevalence (WSW) 43 / 167 (25.7) ?4 ungradeablePrevalence (WSM) 27 / 187 (14.4) ?2 ungradeable Prevalence 96 / 335 (28.7)Persistence 31 / 120 (25.8) Prevalence 14 / 237 (5.9)Hay-Ison criteria AS + NSMarrazzo et al, 2008 [29] Marrazzo et al, 2009 [27]USAObservational cohort Cross-sectionalaaUSANS + PCRb Marrazzo et al, 2010 [18]USAProspective cohort Cross-sectionalPrevalence 96 / 335 (28.7)AS + NS + PCR ASMarrazzo et al, 2010 [20]bbbUSAIncidence 40 / 199 (20.1)Marrazzo et al, 2011 [30]USARandomised control trial Cross-sectionalPersistence 12 / 43 (27.9) intervention arm, Persistence 8 / 38 (21.1) control arm Prevalence 24 / 193 (12.4) c Prevalence 82 / 146 (56.2) c Prevalence 93 / 196 (47.4) Prevalence 125 / 458 (27)AS + NScFethers et al, 2012 [32]AustraliaNSMuzny et al, 2013 [28] Bradshaw et al, 2014 [12] Vodstrcil et al, 2014 [19]aa b cUSA AustraliaCross-sectional Cross-sectional196 WSW, age 18 years 458 WSW, age 18?5 years 298 WSW, age 17?5 yearsAS + NS NSAustraliaLongitudinal cohortIncidence 51/298 (10 per 100PY)NSSub-population (of studya); different variables investigated)Same study population (bbSub-population; different outcomes measured: prevalent, incident and persistent BV) Includes both women who have sex with women (WSW), women who have sex with men (WSM) and women who have sex with women and men(WSWM) doi:10.1371/journal.pone.0141905.tassociated with incident BV in WSW on univariate analysis in one study, but was not included in the multivariate analysis due to SC144 site correlation with other variables.[19] There was no j.jebo.2013.04.005 association between smoking and incident or prevalent BV in three studies from one group,[18,20,29]PLOS ONE | DOI:10.1371/journal.pone.0141905 December 16,5 /Table 2. Variables investigated for association with BV in studies included for review. Blank where the variable not investigated or variable not stratified by WSW alone. Odds ratios/ Risk Ratio/ Hazard Ratio/ Proportions/ Kappa score for correlation of vaginal flora (95 confidence intervals) are displayed (Bold where significant) for factors associated with BV. Variables positively/ negatively associated with BV are from multivariate UNC0642 supplier analyses unless otherwise indicated (P reported proportions only, U univariate analysis only). aSame study population (aaSub-population; differen., ages if reported) 103 WSW, including 21 monogamous couples 91 women Setting Number BV positive/ Total ( ) Diagnostic method(s) ASBerger et al,1995 [13] McCaffrey et al, 1999 [16]a Marrazzo et al,2002 [15]USACross-sectionalGynaecology practice, community clinic Specialist lesbian genitourinary clinic Community recruitmentPrevalence 29 / 101 (28.7) ?2 ungradeable Prevalence 47 / 91 (51.6)UKCross-sectionalHay-Ison criteria NSUSACross-sectional326 WSW, including 58 monogamous couples, age 16 years 708 WSW, age 16?3 years 171 WSW, 189 heterosexual women, age 16?0 years 335 WSW, age 16?0 years jir.2010.0097 237 WSW, age 16 years 335 WSW, age 16?5 years 335 WSW, age 16?5 years 89 WSW, age 16?0 years 193 women, age 17?21 yearsc 146 symptomatic womencPrevalence 81 / 326 (25)Bailey et al, 2004 [31] Evans et al, 2007[14]bbUKCross-sectionalNew patients at a lesbian/ bisexual sexual health clinic Community recruitment Community recruitment Community recruitment Community recruitment Community recruitment Community recruitment University recruitment Sexual health clinic STD Clinic Community recruitment, STI clinic, GP Clinic Community recruitment, STI clinic, GP clinicPrevalence 222 / 708 (31.4)ASUKCross-sectionalPrevalence (WSW) 43 / 167 (25.7) ?4 ungradeablePrevalence (WSM) 27 / 187 (14.4) ?2 ungradeable Prevalence 96 / 335 (28.7)Persistence 31 / 120 (25.8) Prevalence 14 / 237 (5.9)Hay-Ison criteria AS + NSMarrazzo et al, 2008 [29] Marrazzo et al, 2009 [27]USAObservational cohort Cross-sectionalaaUSANS + PCRb Marrazzo et al, 2010 [18]USAProspective cohort Cross-sectionalPrevalence 96 / 335 (28.7)AS + NS + PCR ASMarrazzo et al, 2010 [20]bbbUSAIncidence 40 / 199 (20.1)Marrazzo et al, 2011 [30]USARandomised control trial Cross-sectionalPersistence 12 / 43 (27.9) intervention arm, Persistence 8 / 38 (21.1) control arm Prevalence 24 / 193 (12.4) c Prevalence 82 / 146 (56.2) c Prevalence 93 / 196 (47.4) Prevalence 125 / 458 (27)AS + NScFethers et al, 2012 [32]AustraliaNSMuzny et al, 2013 [28] Bradshaw et al, 2014 [12] Vodstrcil et al, 2014 [19]aa b cUSA AustraliaCross-sectional Cross-sectional196 WSW, age 18 years 458 WSW, age 18?5 years 298 WSW, age 17?5 yearsAS + NS NSAustraliaLongitudinal cohortIncidence 51/298 (10 per 100PY)NSSub-population (of studya); different variables investigated)Same study population (bbSub-population; different outcomes measured: prevalent, incident and persistent BV) Includes both women who have sex with women (WSW), women who have sex with men (WSM) and women who have sex with women and men(WSWM) doi:10.1371/journal.pone.0141905.tassociated with incident BV in WSW on univariate analysis in one study, but was not included in the multivariate analysis due to correlation with other variables.[19] There was no j.jebo.2013.04.005 association between smoking and incident or prevalent BV in three studies from one group,[18,20,29]PLOS ONE | DOI:10.1371/journal.pone.0141905 December 16,5 /Table 2. Variables investigated for association with BV in studies included for review. Blank where the variable not investigated or variable not stratified by WSW alone. Odds ratios/ Risk Ratio/ Hazard Ratio/ Proportions/ Kappa score for correlation of vaginal flora (95 confidence intervals) are displayed (Bold where significant) for factors associated with BV. Variables positively/ negatively associated with BV are from multivariate analyses unless otherwise indicated (P reported proportions only, U univariate analysis only). aSame study population (aaSub-population; differen., ages if reported) 103 WSW, including 21 monogamous couples 91 women Setting Number BV positive/ Total ( ) Diagnostic method(s) ASBerger et al,1995 [13] McCaffrey et al, 1999 [16]a Marrazzo et al,2002 [15]USACross-sectionalGynaecology practice, community clinic Specialist lesbian genitourinary clinic Community recruitmentPrevalence 29 / 101 (28.7) ?2 ungradeable Prevalence 47 / 91 (51.6)UKCross-sectionalHay-Ison criteria NSUSACross-sectional326 WSW, including 58 monogamous couples, age 16 years 708 WSW, age 16?3 years 171 WSW, 189 heterosexual women, age 16?0 years 335 WSW, age 16?0 years jir.2010.0097 237 WSW, age 16 years 335 WSW, age 16?5 years 335 WSW, age 16?5 years 89 WSW, age 16?0 years 193 women, age 17?21 yearsc 146 symptomatic womencPrevalence 81 / 326 (25)Bailey et al, 2004 [31] Evans et al, 2007[14]bbUKCross-sectionalNew patients at a lesbian/ bisexual sexual health clinic Community recruitment Community recruitment Community recruitment Community recruitment Community recruitment Community recruitment University recruitment Sexual health clinic STD Clinic Community recruitment, STI clinic, GP Clinic Community recruitment, STI clinic, GP clinicPrevalence 222 / 708 (31.4)ASUKCross-sectionalPrevalence (WSW) 43 / 167 (25.7) ?4 ungradeablePrevalence (WSM) 27 / 187 (14.4) ?2 ungradeable Prevalence 96 / 335 (28.7)Persistence 31 / 120 (25.8) Prevalence 14 / 237 (5.9)Hay-Ison criteria AS + NSMarrazzo et al, 2008 [29] Marrazzo et al, 2009 [27]USAObservational cohort Cross-sectionalaaUSANS + PCRb Marrazzo et al, 2010 [18]USAProspective cohort Cross-sectionalPrevalence 96 / 335 (28.7)AS + NS + PCR ASMarrazzo et al, 2010 [20]bbbUSAIncidence 40 / 199 (20.1)Marrazzo et al, 2011 [30]USARandomised control trial Cross-sectionalPersistence 12 / 43 (27.9) intervention arm, Persistence 8 / 38 (21.1) control arm Prevalence 24 / 193 (12.4) c Prevalence 82 / 146 (56.2) c Prevalence 93 / 196 (47.4) Prevalence 125 / 458 (27)AS + NScFethers et al, 2012 [32]AustraliaNSMuzny et al, 2013 [28] Bradshaw et al, 2014 [12] Vodstrcil et al, 2014 [19]aa b cUSA AustraliaCross-sectional Cross-sectional196 WSW, age 18 years 458 WSW, age 18?5 years 298 WSW, age 17?5 yearsAS + NS NSAustraliaLongitudinal cohortIncidence 51/298 (10 per 100PY)NSSub-population (of studya); different variables investigated)Same study population (bbSub-population; different outcomes measured: prevalent, incident and persistent BV) Includes both women who have sex with women (WSW), women who have sex with men (WSM) and women who have sex with women and men(WSWM) doi:10.1371/journal.pone.0141905.tassociated with incident BV in WSW on univariate analysis in one study, but was not included in the multivariate analysis due to correlation with other variables.[19] There was no j.jebo.2013.04.005 association between smoking and incident or prevalent BV in three studies from one group,[18,20,29]PLOS ONE | DOI:10.1371/journal.pone.0141905 December 16,5 /Table 2. Variables investigated for association with BV in studies included for review. Blank where the variable not investigated or variable not stratified by WSW alone. Odds ratios/ Risk Ratio/ Hazard Ratio/ Proportions/ Kappa score for correlation of vaginal flora (95 confidence intervals) are displayed (Bold where significant) for factors associated with BV. Variables positively/ negatively associated with BV are from multivariate analyses unless otherwise indicated (P reported proportions only, U univariate analysis only). aSame study population (aaSub-population; differen., ages if reported) 103 WSW, including 21 monogamous couples 91 women Setting Number BV positive/ Total ( ) Diagnostic method(s) ASBerger et al,1995 [13] McCaffrey et al, 1999 [16]a Marrazzo et al,2002 [15]USACross-sectionalGynaecology practice, community clinic Specialist lesbian genitourinary clinic Community recruitmentPrevalence 29 / 101 (28.7) ?2 ungradeable Prevalence 47 / 91 (51.6)UKCross-sectionalHay-Ison criteria NSUSACross-sectional326 WSW, including 58 monogamous couples, age 16 years 708 WSW, age 16?3 years 171 WSW, 189 heterosexual women, age 16?0 years 335 WSW, age 16?0 years jir.2010.0097 237 WSW, age 16 years 335 WSW, age 16?5 years 335 WSW, age 16?5 years 89 WSW, age 16?0 years 193 women, age 17?21 yearsc 146 symptomatic womencPrevalence 81 / 326 (25)Bailey et al, 2004 [31] Evans et al, 2007[14]bbUKCross-sectionalNew patients at a lesbian/ bisexual sexual health clinic Community recruitment Community recruitment Community recruitment Community recruitment Community recruitment Community recruitment University recruitment Sexual health clinic STD Clinic Community recruitment, STI clinic, GP Clinic Community recruitment, STI clinic, GP clinicPrevalence 222 / 708 (31.4)ASUKCross-sectionalPrevalence (WSW) 43 / 167 (25.7) ?4 ungradeablePrevalence (WSM) 27 / 187 (14.4) ?2 ungradeable Prevalence 96 / 335 (28.7)Persistence 31 / 120 (25.8) Prevalence 14 / 237 (5.9)Hay-Ison criteria AS + NSMarrazzo et al, 2008 [29] Marrazzo et al, 2009 [27]USAObservational cohort Cross-sectionalaaUSANS + PCRb Marrazzo et al, 2010 [18]USAProspective cohort Cross-sectionalPrevalence 96 / 335 (28.7)AS + NS + PCR ASMarrazzo et al, 2010 [20]bbbUSAIncidence 40 / 199 (20.1)Marrazzo et al, 2011 [30]USARandomised control trial Cross-sectionalPersistence 12 / 43 (27.9) intervention arm, Persistence 8 / 38 (21.1) control arm Prevalence 24 / 193 (12.4) c Prevalence 82 / 146 (56.2) c Prevalence 93 / 196 (47.4) Prevalence 125 / 458 (27)AS + NScFethers et al, 2012 [32]AustraliaNSMuzny et al, 2013 [28] Bradshaw et al, 2014 [12] Vodstrcil et al, 2014 [19]aa b cUSA AustraliaCross-sectional Cross-sectional196 WSW, age 18 years 458 WSW, age 18?5 years 298 WSW, age 17?5 yearsAS + NS NSAustraliaLongitudinal cohortIncidence 51/298 (10 per 100PY)NSSub-population (of studya); different variables investigated)Same study population (bbSub-population; different outcomes measured: prevalent, incident and persistent BV) Includes both women who have sex with women (WSW), women who have sex with men (WSM) and women who have sex with women and men(WSWM) doi:10.1371/journal.pone.0141905.tassociated with incident BV in WSW on univariate analysis in one study, but was not included in the multivariate analysis due to correlation with other variables.[19] There was no j.jebo.2013.04.005 association between smoking and incident or prevalent BV in three studies from one group,[18,20,29]PLOS ONE | DOI:10.1371/journal.pone.0141905 December 16,5 /Table 2. Variables investigated for association with BV in studies included for review. Blank where the variable not investigated or variable not stratified by WSW alone. Odds ratios/ Risk Ratio/ Hazard Ratio/ Proportions/ Kappa score for correlation of vaginal flora (95 confidence intervals) are displayed (Bold where significant) for factors associated with BV. Variables positively/ negatively associated with BV are from multivariate analyses unless otherwise indicated (P reported proportions only, U univariate analysis only). aSame study population (aaSub-population; differen.