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S Heterodera glycines. In separate investigations, In the fall of 2011, cysts related with buy Chrysophanic acid Amorpha fruiticosa L., frequently generally known as false indigo-bush, were identified as Cactodera betulae utilizing both morphological and molecular methods. That is a new host for this nematode. The nematode (C. betulae) had been identified as early as 1989 within this area along the White River near Beulah, AR by D.G. Kim, but efforts to recognize it in the time have been unsuccessful. This nematode has also been was reported in association with black locust (Robinia pseudoacacia) inside the Fayetteville, AR vicinity by R. D. Riggs. Amorpha fruiticosa is often a new host for C. betulae. Species identification of each H. urticae and C. betulae had been confirmed by molecular analyses in the near-full-length modest subunit rDNA gene, D2/D3 expansion segments of the huge subunit rDNA gene and internal transcribed spacer. HIGH-THROUGHPUT GENOTYPING IN GENETIC MAPPING AND BREEDING FOR ROOT-KNOT NEMATODE RESISTANCE. Roberts, Philip A., L.-B. Huynh, S. Wannamaker, J.E. Ehlers and T.J. Close. University of California, Riverside, CA 92521. Conventional breeding approaches PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/20060508 to create root-knot nematode (Meloidogyne spp.) resistant crop plants are generally inefficient resulting from time and price requirements for phenotyping progenies for resistance response. Screens for resistance rely on assays of nematode reproduction or indices of root-gall symptoms and might be unreliable even beneath controlled circumstances. Resistance choice improvements lie in application of molecular marker and large-scale genotyping approaches for indirect selection of resistance loci. Within the big crops which include corn, cotton, and soybean, private sector breeding is applying these systems, but in public programs these innovations are typically unavailable. We created a complete molecular breeding (MB) method for cowpea (Vigna unguiculata), a vital grain legume and fodder crop. Three root-knot nematode species (M. incognita, M. javanica, M. arenaria) are highly damaging to cowpea straight and as components of a illness complex with Fusarium wilt (Fusarium oxysporum f. sp. tracheiphilum), even so effective resistance genes for these pathogens are offered for cultivar improvement. A genotyping platform was created with genome-wide EST-derived SNP markers identified from 17 cDNA libraries, 1st as an Illumina 1536-SNP GoldenGate genotyping assay and subsequently as a more versatile KBioscience KASPar method. The genotyping assay was applied to 12 RIL populations to construct person and consensus genetic maps incorporating 1107 markers. RIL populations segregating for resistance were phenotyped in field and growth-pouch screens and resistance loci located by QTL mapping. The genome-wide SNP marker coverage enables both foreground (trait-based) and background selection, combining targeted resistance loci with each other with genome regions carrying favorable development, yield as well as other stress tolerance traits. The high-throughput capability allows substantial numbers of individuals488 Journal of Nematology, Volume 44, No. four, December 2012 to be genotyped simultaneously at hundreds of loci for marker-assisted backcrossing (MABC) and recurrent choice (MARS) approaches in MB programs. A configurable workflow for applying MB tools of this method becoming developed as an Integrated Breeding Platform by the CGIAR- Generation Challenge System will be discussed. GRAPEVINE NEMATODE MANAGEMENT WITH PAECILOMYCES LILACINUS On the CENTRAL CALIFORNIA.