Of the enzyme. Using ChIP assay, strong H3-K4 trimethylation in

Of the enzyme. Using ChIP assay, strong H3-K4 trimethylation in the 15-LOX-1 promoter region of L1236 cells was observed. In contrast, this region was markedly less methylated in L428 cells (Fig. 1C). The results suggest an association of 15-LOX-1 promoter histone methylation status with transcriptional status of the gene in cultured HL cells.Histone Methyltransferase SMYD3 and Histone Demethylase SMCX GMX1778 Regulate 15-LOX-1 Gene ExpressionBecause 15-LOX-1 expression is closely related to H3-K4 methylation status, we next determined whether H3-K4 trimethylation is required for 15-LOX-1 transcription. Inhibition of SMYD3, a histone H3-K4 pecific dimethyltransferase and trimethyltransferase, could lead to a hypo-methylated status of H3-K4 at the promoter region of its target genes [24,33]. As shown in Fig. 2A and Fig. 3 A, B, knocking down SMYD3 expression using siRNA significantly inhibits H3-K4 methylation at the 15-LOX-1 promoter region and reduces 15-LOX-1 mRNA abundance in L1236. Expression of SMYD3 protein is undetectable in L428 cells (data not shown). To further test the hypothesis that 15-LOX-1 expression is controlled by the histone methylation status, the SMCX gene, coding a H3-K4 demethylase [34], was silenced in L428 cells by means of siRNA. The analyses showed that H3-K4 turned hyper-methylated at the 15-LOX-1 promoter and that the expression of the enzyme was induced at both mRNASMYD3 is Physically Associated with the 15-LOX-1 PromoterSince the SMYD3 binding motif was shown to be involved in 15-LOX-1 transcription and SMYD3 regulates 15-LOX-1 promoter activity, we asked whether SMYD3 binds to the 15-LOX-1 promoter region in vivo in L1236 cells. To this end, ChIP assay was applied with primers encompassing the SMYD3 binding motif (Fig. 3A). We found that the 15-LOX-1 core promoter region covering the SMYD3 binding motif is occupied by SMYD3 in vivo and that the association was inhibited when SMYD3 was knocked down by siRNA (Fig. 3B). The specificity of the association was verified by the absence of specific sequence amplifications when antibodies were omitted and when primers for the unrelated GAPDH gene were applied in the PCR reaction.Histone Methylation Regulates 15-LOX-1 ExpressionFigure 1. Association of 15-LOX-1 expression and activity with H3-K4 methylation status in HL-derived cell lines. (A) 15-LOX-1 mRNA levels in L1236 and L428 cells were measured using real-time PCR (n = 6). The expression levels were calculated relative to that of a calibrator sample (A549 cells ectopically expressing 15-LOX-1), levels of transcripts were expressed as the ratio vs. human b2 microglobulin. Bar, SD; ** p,0.01. (B) 15HETE was measured as an indication of 15-LOX-1 activity in L1236 and L428 cells (n = 4). Cells were harvested after incubation with exogenous arachidonic acid (40 mM) at 37uC for 5 min. Bar, SD; 15826876 * p,0.05. (C) ChIP assay for H3-K4 trimethylation at the 15-LOX-1 core promoter in L1236 and L428 cells. Primers B (see Materials and Methods and Fig. 3 A) were used for PCR amplification. Shown is one of four independent experiments. doi:10.1371/journal.pone.0052703.gFigure 2. SMYD3 and SMCX regulate 15-LOX-1 expression in cultured HL-derived cells cells and prostate cancer cells. (A) Real-time PCR assay of 15-LOX-1 mRNA expression in L1236 and LNCaP cells treated with SMYD3 siRNAs or control siRNA (n = 4). The results were ASP2215 normalized to the mRNA level of beta-2 microglobulin. The efficiency of SMYD3 siRNA knocking down was evaluat.Of the enzyme. Using ChIP assay, strong H3-K4 trimethylation in the 15-LOX-1 promoter region of L1236 cells was observed. In contrast, this region was markedly less methylated in L428 cells (Fig. 1C). The results suggest an association of 15-LOX-1 promoter histone methylation status with transcriptional status of the gene in cultured HL cells.Histone Methyltransferase SMYD3 and Histone Demethylase SMCX Regulate 15-LOX-1 Gene ExpressionBecause 15-LOX-1 expression is closely related to H3-K4 methylation status, we next determined whether H3-K4 trimethylation is required for 15-LOX-1 transcription. Inhibition of SMYD3, a histone H3-K4 pecific dimethyltransferase and trimethyltransferase, could lead to a hypo-methylated status of H3-K4 at the promoter region of its target genes [24,33]. As shown in Fig. 2A and Fig. 3 A, B, knocking down SMYD3 expression using siRNA significantly inhibits H3-K4 methylation at the 15-LOX-1 promoter region and reduces 15-LOX-1 mRNA abundance in L1236. Expression of SMYD3 protein is undetectable in L428 cells (data not shown). To further test the hypothesis that 15-LOX-1 expression is controlled by the histone methylation status, the SMCX gene, coding a H3-K4 demethylase [34], was silenced in L428 cells by means of siRNA. The analyses showed that H3-K4 turned hyper-methylated at the 15-LOX-1 promoter and that the expression of the enzyme was induced at both mRNASMYD3 is Physically Associated with the 15-LOX-1 PromoterSince the SMYD3 binding motif was shown to be involved in 15-LOX-1 transcription and SMYD3 regulates 15-LOX-1 promoter activity, we asked whether SMYD3 binds to the 15-LOX-1 promoter region in vivo in L1236 cells. To this end, ChIP assay was applied with primers encompassing the SMYD3 binding motif (Fig. 3A). We found that the 15-LOX-1 core promoter region covering the SMYD3 binding motif is occupied by SMYD3 in vivo and that the association was inhibited when SMYD3 was knocked down by siRNA (Fig. 3B). The specificity of the association was verified by the absence of specific sequence amplifications when antibodies were omitted and when primers for the unrelated GAPDH gene were applied in the PCR reaction.Histone Methylation Regulates 15-LOX-1 ExpressionFigure 1. Association of 15-LOX-1 expression and activity with H3-K4 methylation status in HL-derived cell lines. (A) 15-LOX-1 mRNA levels in L1236 and L428 cells were measured using real-time PCR (n = 6). The expression levels were calculated relative to that of a calibrator sample (A549 cells ectopically expressing 15-LOX-1), levels of transcripts were expressed as the ratio vs. human b2 microglobulin. Bar, SD; ** p,0.01. (B) 15HETE was measured as an indication of 15-LOX-1 activity in L1236 and L428 cells (n = 4). Cells were harvested after incubation with exogenous arachidonic acid (40 mM) at 37uC for 5 min. Bar, SD; 15826876 * p,0.05. (C) ChIP assay for H3-K4 trimethylation at the 15-LOX-1 core promoter in L1236 and L428 cells. Primers B (see Materials and Methods and Fig. 3 A) were used for PCR amplification. Shown is one of four independent experiments. doi:10.1371/journal.pone.0052703.gFigure 2. SMYD3 and SMCX regulate 15-LOX-1 expression in cultured HL-derived cells cells and prostate cancer cells. (A) Real-time PCR assay of 15-LOX-1 mRNA expression in L1236 and LNCaP cells treated with SMYD3 siRNAs or control siRNA (n = 4). The results were normalized to the mRNA level of beta-2 microglobulin. The efficiency of SMYD3 siRNA knocking down was evaluat.