To reduce in-flow artifacts. Secondly, the retrospective-gated CINE sequence covered the

To reduce in-flow artifacts. Secondly, the retrospective-gated CINE sequence covered the full cardiac cycle without dead time at the end of the cardiac cycle waiting for the next ECG C.I. 19140 trigger. Using the retrospective-gated CINE sequence in combination with contrast agents known to accumulate in atherosclerotic plaques, micelles as well as USPIO, allowed for a good discrimination of the atherosclerotic lesion on the inner curvature of the aortic arch [9,10].Plaque Burden and Aortic StiffnessThe finding of age-related increases in aortic stiffness, vessel diameter and aortic atherosclerosis is consistent with prior studies [36]. Traditionally, the stiffness and compliance of conduit vessels is an estimate from the pulse wave velocity (PWV) [37,38]. This is routinely done in the clinic using ultrasound or MRI, yet PWV measurements in mice are feasible as shown in the group of Jakob, but challenging due to the high heart rate and difficulties to measure flow velocities in vivo [15,16]. Using the stiffness of the vessel wall can be a good alternative or additional tool to characterize vessel compliance. The correlation between aortic stiffness and plaque burden is particularly interesting because the elastic properties of the aortic wall play an important role in the pathogenesis of cardiovascular disease including atherosclerosis and hypertension, and is an independent risk factor for ventricular hypertrophy and stroke. Further pathophysiological studies may include longitudinal follow-up experiments to assess the temporal relationship between vascular compliance and plaque burden as well as the ageing related increase in vessel wall diameter. Our study showed that in a ApoE2/2 mouse model the histological plaque burden was closely related to both contrastenhancement on MRI and the aortic distensibility. This correlation was preserved over a large age range, also during statin treatment, indicating that in this mouse model, changes in aortic stiffness are dominated by the plaque burden. The average circumferential strain decreases with age, Eliglustat whereas statin treatment slows down this decrease. Together with the observation of an increase in vessel wall diameter this points at a decrease in arterial elasticity and compliance with age and in relation to the extent of atherosclerosis progression. Therefore, aortic compliance measurements may be an alternative approach in this animal model to monitor subtle changes of the arterial wall elasticity to actual plaque imaging, which is still difficult and time-consuming. Aortic circumferential strain measurements would provide a straight-forward method to study the response to various dietary and pharmacological manipulations both in this animal models and patients [39,40]. Though we found a very clear correlation between aortic stiffness and plaque burden in the ApoE2/2 mouse, this is not necessarily the case in other mouse models, nor in patients. Arterial stiffness is an independent predictor of ventricular hypertrophy and stroke in patients, indicating that other mechanisms than plaque development may cause stiffening of the arteries. Assessing the temporal relationship between vascular compliance and plaque burden, may be particularly useful in different mouse models of atherosclerosis, including models of vascular dysfunction.Discussion Self-Gated CINE MRI for Atherosclerotic Plaque DetectionAtherosclerotic plaque formation typically originates in the aortic root and progresses to the ascending a.To reduce in-flow artifacts. Secondly, the retrospective-gated CINE sequence covered the full cardiac cycle without dead time at the end of the cardiac cycle waiting for the next ECG trigger. Using the retrospective-gated CINE sequence in combination with contrast agents known to accumulate in atherosclerotic plaques, micelles as well as USPIO, allowed for a good discrimination of the atherosclerotic lesion on the inner curvature of the aortic arch [9,10].Plaque Burden and Aortic StiffnessThe finding of age-related increases in aortic stiffness, vessel diameter and aortic atherosclerosis is consistent with prior studies [36]. Traditionally, the stiffness and compliance of conduit vessels is an estimate from the pulse wave velocity (PWV) [37,38]. This is routinely done in the clinic using ultrasound or MRI, yet PWV measurements in mice are feasible as shown in the group of Jakob, but challenging due to the high heart rate and difficulties to measure flow velocities in vivo [15,16]. Using the stiffness of the vessel wall can be a good alternative or additional tool to characterize vessel compliance. The correlation between aortic stiffness and plaque burden is particularly interesting because the elastic properties of the aortic wall play an important role in the pathogenesis of cardiovascular disease including atherosclerosis and hypertension, and is an independent risk factor for ventricular hypertrophy and stroke. Further pathophysiological studies may include longitudinal follow-up experiments to assess the temporal relationship between vascular compliance and plaque burden as well as the ageing related increase in vessel wall diameter. Our study showed that in a ApoE2/2 mouse model the histological plaque burden was closely related to both contrastenhancement on MRI and the aortic distensibility. This correlation was preserved over a large age range, also during statin treatment, indicating that in this mouse model, changes in aortic stiffness are dominated by the plaque burden. The average circumferential strain decreases with age, whereas statin treatment slows down this decrease. Together with the observation of an increase in vessel wall diameter this points at a decrease in arterial elasticity and compliance with age and in relation to the extent of atherosclerosis progression. Therefore, aortic compliance measurements may be an alternative approach in this animal model to monitor subtle changes of the arterial wall elasticity to actual plaque imaging, which is still difficult and time-consuming. Aortic circumferential strain measurements would provide a straight-forward method to study the response to various dietary and pharmacological manipulations both in this animal models and patients [39,40]. Though we found a very clear correlation between aortic stiffness and plaque burden in the ApoE2/2 mouse, this is not necessarily the case in other mouse models, nor in patients. Arterial stiffness is an independent predictor of ventricular hypertrophy and stroke in patients, indicating that other mechanisms than plaque development may cause stiffening of the arteries. Assessing the temporal relationship between vascular compliance and plaque burden, may be particularly useful in different mouse models of atherosclerosis, including models of vascular dysfunction.Discussion Self-Gated CINE MRI for Atherosclerotic Plaque DetectionAtherosclerotic plaque formation typically originates in the aortic root and progresses to the ascending a.