Ng default parameters and 1000 bootstraps with RAxML v8.two.12 [49]. The 16s rRNANg default parameters

Ng default parameters and 1000 bootstraps with RAxML v8.two.12 [49]. The 16s rRNA
Ng default parameters and 1000 bootstraps with RAxML v8.two.12 [49]. The 16s rRNA gene of Staphylococcus aureus (RefSeq ID: GCF_000013425.1) was made use of as an outgroup. The origin of replication (OriC) was identified applying DoriC database [50] and Mauve aligner [51]. Pairwise genomic comparison of strain BSE6.1 was made with three other associated genomes. Dotplots have been constructed with minimap2 based pairwise alignment utilizing D-Genies [52]. Prokka v1.14.6 was made use of to perform a local de novo annotation [53]. Pan-genome comparison with one hundred related genomes ( 90 16S nucleotide identity; 80 whole-genome aligned fraction identity) was produced using the pan-genome tool at KBase server [46]. Gene clusters related for the secondary metabolite biosynthesis had been identified using the antiSMASH 5.0 pipeline [54]. The red pigmentproducing gene cluster of BSE6.1 was compared with that of S. coelicolor A3(two), Serratia, and Hahella using the multigene BLAST tool [55]. The distribution of a variety of coding sequences (CDS) and gene clusters across the genome was IDO review plotted applying Circos [56].Microorganisms 2021, 9, x FOR PEER REVIEW4 ofMicroorganisms 2021, 9,A3(two), Serratia, and Hahella working with the multigene BLAST tool [55]. The distribution17 vari4 of of ous coding sequences (CDS) and gene clusters across the genome was plotted utilizing Circos [56].Figure 1. Workflow and pipeline of toolsand pipeline of tools used reads into a genome reads into a genome and further Figure 1. Workflow made use of to assemble the raw to assemble the raw and further analysis on the assembled genome. analysis on the assembled genome.three. Final results and Discussion Strain BSE6.1 developed a pink-colored development in Minimal broth with 2 NaCl and red pigmentation in all other compatible media. Pale pink to reddish colonies with whiteMicroorganisms 2021, 9, x FOR PEER REVIEW5 of3. Final results and DiscussionMicroorganisms 2021, 9,Strain BSE6.1 made a pink-colored development in Minimal broth with two NaCl and red pigmentation in all other compatible media. Pale pink to reddish colonies with white powdery BRD7 review spores have been observed right after 7 or ten days of incubation. Salt tolerance was observed up to a rangeobserved right after 7 orbacterium incubation. Salt tolerance was observed powdery spores have been of two to 7 . This ten days of was positive for catalase and oxidase activities. In our earlier study, strain BSE6.1 showed potential antibacterial activity against up to a range of 2 to 7 . This bacterium was positive for catalase and oxidase activities. distinctive human pathogens and also displayed a robust potential toactivity against distinctive In our earlier study, strain BSE6.1 showed potential antibacterial stain epidermis and parenchyma cells of Tridax procumbens stem [25]. The maximum pigmentand parenchyma human pathogens and also displayed a powerful ability to stain epidermis production was observed at 29procumbens stem [25]. The maximum pigmentfor its development was 38 (Figcells of Tridax , plus the maximum temperature tolerance production was observed at ure2). plus the maximum temperature on the red for its growth was 38 Cobserved2). The 29 C, The peak absorption spectrum tolerance pigment of BSE6.1 was (Figure at 528 nm [25]. peak absorption spectrum from the red pigment of BSE6.1 was observed at 528 nm [25].five ofFigure Morphological and biochemical Figure 2. Morphological and biochemical characteristics of Streptomyces sp. strain BSE6.1.Identification in the red pigment by means of thin layer chromatography (TLC), FourierIdentification of the red.