ntributions NM, GJD, HSO, and JGB designed and planned the analysis. MH prepared fungal cultures.

ntributions NM, GJD, HSO, and JGB designed and planned the analysis. MH prepared fungal cultures. CB and SS ready activitybased c-Raf drug probes utilized in this study. NM collected secretome samples and performed activitybased protein profil ing experiments. NM collected and analysed proteomic data. DN performed bioinformatic analysis. NM and MS ready P. pastoris strains, produced and purified recombinant enzymes, and performed activity assays. NM wrote the manuscript with input from each of the authors. All authors study and approved the final manuscript. Funding The authors thank the Natural Sciences and Engineering Analysis Council of Canada (PostDoctoral Fellowship to NGSM), the Royal Society (Ken Murray Study Professorship to GJD), the Biotechnology and Biological Sciences Study Council (BBSRC) (grant BB/R001162/1 to GJD), the French National Analysis Agency (ANR13BIME0002 to JGB), the Netherlands Organization for Scientific Analysis (NWO Best grant 2018714.018.002 to HSO), and also the European Research Council (ERC2011AdG290836 “Chembiosphing” to HSO, ERC2020SyG951231 “Carbocentre” to GJD and HSO). Proteomics information had been collected in the York Centre of Excellence in Mass Spectrometry, which was created due to a significant capital investment via Science City York, sup ported by Yorkshire Forward with funds from the Northern Way Initiative, and subsequent support from EPSRC (EP/K039660/1; EP/M028127/1). Availability of information and supplies Pichia pastoris strains and samples of recombinant proteins could be accessible from Gideon Davies ([email protected]). Samples of ABPCel, ABPXyl, and ABPGlc may possibly be offered from Herman Overkleeft (h.s.overkleeft@lic. leidenuniv.nl). Basidiomycete fungi are out there from the fungal culture collection of your International Centre of Microbial Sources (CIRMCF) in the French National Institute for Agricultural research (INRA; Marseille, France). Genome sequences for each in the fungi made use of within this study are available from Mycocosm (mycocosm.jgi.doe.gov/mycocosm/home) (DOE Joint Genome Institute, Walnut Creek, California). Other datasets utilized and/or ana lysed during the existing study are available from the corresponding author on affordable request.Author details 1 York Structural Biology Laboratory, Department of Chemistry, The University of York, Heslington YO10 5DD, York, UK. two CCKBR Gene ID Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA Leiden, The Netherlands. 3 UMR1163 Bio diversitet Biotechnologie Fongiques, Facultdes Sciences de Luminy, INRAE, Aix Marseille Univ, 13288 Marseille, France. four Polytech Marseille, Aix Marseille Univ, 13288 Marseille, France. Received: eight October 2021 Accepted: six JanuaryDeclarationsEthics approval and consent to participate Not applicable. Consent for publication Not applicable. Competing interests The authors declare no competing interests.References 1. Scheller HV, Ulvskov P. Hemicelluloses. Annu Rev Plant Biol. 2010;two(61):2639. 2. Luis AS, Briggs J, Zhang X, Farnell B, Ndeh D, Labourel A, et al. Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018;3(2):210. 3. Celiska E, Nicaud JM, Bialas W. Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide resources: evaluation on starch, cellulose, xylan, and inulin. Appl Microbiol Biotechnol. 2021;105(three):9759. four. Schlembach I, Hosseinpour Tehrani H, Blank LM, B hs J, Wierckx N, Regestein L, et al. Consolidate