with soil samples from agriculturally in the M sterland M sterland region. Errorindicate regular deviation

with soil samples from agriculturally in the M sterland M sterland region. Errorindicate regular deviation (n = three). (B)(n =base (B) MS base peak chromatogramsupernatant of a soil slurry incubated soil bars indicate normal deviation MS 3). peak chromatogram in the extracted with the extracted supernatant of a with 1 mM cholate 1 mM cholate(top) about 48 h (best) asion chromatograms withchromatograms using the (383 Da slurry incubated with for about 48 h for as well as extracted properly as extracted ion the m/z values of HOCDA m/z values for [M-H]-1, middle) and DOCDA (XX, 385 Da for [M-H]-1, bottom). Samples have been measured in adverse MS mode. (C) 3D of HOCDA (383 Da for [M-H]-1 , middle) and DOCDA (XX, 385 Da for [M-H]-1 , bottom). Samples have been measured in UV chromatogram of the extracted supernatant of a soil slurry incubated with 1 mM cholate for about 48 h and structure damaging MS mode. many intermediates assigned to peaks. Intensity is shown as aa soilmap. Red indicates with 1 mM cholate recommendations for (C) 3D UV chromatogram from the extracted supernatant of heat slurry incubated highest absorpfor about (D)h and structure ideas for many in (B,C). Massesassigned to peaks. Intensity) is shown as a heat map. tion. 48 Candidate structures for peaks a-i located intermediates and absorption maxima (max have been determined by Chk2 Inhibitor Synonyms HPLC-MS measurements. Structure recommendations are primarily based for peaks a-i discovered absorption spectra, and retention maxima Red indicates highest absorption. (D) Candidate structures on molecular masses, in (B,C). Masses and absorptiontime. 1,four 4,6 (maxCandidate structures by HPLC-MS measurements. Structure for the -pathway, and on molecular masses, absorption ) have been determined belonging (blue) to the -pathway, (red) recommendations are primarily based (black) potentially occurring in both pathways. When structures could not be assigned unambiguously, 1,four probable structures are4,six two HSP70 Inhibitor drug depicted. XV: 7,12spectra, and retention time. Candidate structures belonging (blue) towards the -pathway, (red) to the -pathway, and (black) Dihydroxy-3-oxo-pregna-4-ene-carboxylate, XVI: 7-Hydroxy-3,12-dioxo-pregna-4-ene-carboxylate, XVII: 7,12-Dihydroxypotentially occurring in both pathways. When structures could XIX: be assigned unambiguously, two achievable structures are 3-oxo-pregna-4-ene-carboxylate, XVIII: 4-3,12-Diketocholate, not DOCDA (12-Hydroxy-3-oxo-pregna-4,6-diene-carboxdepicted. XV: 7,12-Dihydroxy-3-oxo-pregna-4-ene-carboxylate, XVI: 7-Hydroxy-3,12-dioxo-pregna-4-ene-carboxylate, XVII: ylate, XX: three,12-Dioxo-4,6-choldienoate). 7,12-Dihydroxy-3-oxo-pregna-4-ene-carboxylate, XVIII: four -3,12-Diketocholate, XIX: DOCDA (12-Hydroxy-3-oxo-pregna-4,64. Discussion diene-carboxylate, XX: three,12-Dioxo-4,6-choldienoate). Aerobic bacterial degradation of 7-hydroxy bile salts in soil and water can proceed through two pathway variants, namely the 1,4-variant along with the 4,6-variant [6]. The four,6-variantMicroorganisms 2021, 9,15 of4. Discussion Aerobic bacterial degradation of 7-hydroxy bile salts in soil and water can proceed by means of two pathway variants, namely the 1,four -variant and the 4,six -variant [6]. The four,6 variant is prevalent in the Sphingomonadaceae and differs from the 1,4 -variant, which can be found in other Proteobacteria and Actinobacteria, specifically in the degradation in the side chain [11,23], though the cleavage with the steroid skeleton was proposed to proceed by way of 9,10-seco cleavage in both variants. In Sphingobium sp. strain Chol11, DHSATD (XI) is the expected 9,