(1113 significantly upregulated and 956 significan downregulated), 334 DEGs (155 significantly upregulated and 179 considerably

(1113 significantly upregulated and 956 significan downregulated), 334 DEGs (155 significantly upregulated and 179 considerably dow regulated), 321 DEGs (131 considerably upregulated and 190 substantially downreguAnimals 2021, 11, x FOR PEER REVIEW6 ofAnimals 2021, 11,ed), and 1423 DEGs (582 significantly upregulated and 841 substantially downregulated), respectively. By analyzing a Venn diagram (Figure 4G), it really is evident that there had been 63, 329, 1678, 137, 119, and 970 exclusive differentially expressed genes inside the duodenum, jejunum, ileum, cecum, colon, and rectum, respectively.6 ofA.B.C.D.E.F.G.H.Figure three. Differential gene volcano map, differential gene Wayne map, and differential gene number histogram. (A ) The Vocalo diagram evaluation of DEGs in the duodenum, jejunum, ileum, cecum, colon, and rectum in turn. Red indicates drastically upregulated DEG, and blue indicates substantially downregulated DEG (|log2(foldchange)| 1 and p-value 0.05). (G) Differential gene Venn diagram; diverse colors indicate various comparison combinations. (H) The histogram on the variety of differentially expressed genes is shown in blue and gray. The quantity around the column κ Opioid Receptor/KOR drug represents the number of differentially expressed genes. S_Z: the duodenum of wholesome rabbits, S_B: diarrhea within the duodenum of rabbits, H_Z: wholesome rabbit ileum, H_B: diarrheal rabbit ileum, K_Z: wholesome rabbit jejunum, K_B: rabbits with diarrheal jejunum, M_Z: healthier cecum of rabbits, M_B: rabbits with diarrheal cecum, J_Z: healthy rabbit colon, J_B: colon of rabbits with diarrhea, Z_Z: healthy rabbit rectum, Z_B: rectum of rabbits with diarrhea.36.Yunpeng, C.; Shihua, P.; Jianjun, Z.; Weijie, Z.; Huijuan, N.; Zhicai, Q.; Jingwen, W.; Daleng, S.; Zengji, Y. RAPD Evaluation of ALK4 Inhibitor manufacturer Genetic Relationships among Natural Populations of Hybrid Taxodium Mucronatum Tenore. Fu Dan Xue Bao Zi Ran Ke Xue Ban J. Fudan Univ. Nat. Sci. 2002, 41, 64145.Animals 2021, 11,7 ofBiosensors 2021, 11, x FOR PEER REVIEW13 ofFigure 4. Cont.Animals 2021, 11,8 ofFigure 4. GO enrichment analysis of DEGs in unique comparison groups. S_Z vs. S_B (A), K_Z vs. K_B (B), H_Z vs. H_B (C), M_Z vs. M_B (D), J_Z vs. J_B (E), and Z_Z vs. Z_B (F). GO terms are on the x-axis. The enrichment ratio of genes is shown as GO terms for BP, CC, and MF. S_Z: the duodenum of healthier rabbits, S_B: diarrhea within the duodenum of rabbits, H_Z: wholesome rabbit ileum, H_B: rabbit with diarrheal ileum, K_Z: wholesome rabbit jejunum, K_B: rabbits with diarrheal jejunum, M_Z: healthful cecum of rabbits, M_B: rabbits with diarrheal cecum, J_Z: wholesome rabbit colon, J_B: colon of rabbits with diarrhea, Z_Z: wholesome rabbit rectum, Z_B: rectum of rabbits with diarrhea.three.five. Enrichment Analysis of GO and KEGG Pathway Compared using the basic description of your properties of genes or transcripts with functional annotation, the lowest degree of gene function and KEGG pathway might be annotated by enrichment analyses. Also, enrichment analysis delivers essentially the most detailed information regarding gene function and KEGG pathway, which can help us to screen exceptional insights on diarrhea response in rabbits fed a no-antibiotic diet plan. Moreover, GO terms and KEGG pathways that satisfy the corrected p-value of 0.05 were viewed as considerably enriched. To classify and characterize DEG functions and pathways, we performed a gene ontology (GO) classification and functional annotation of molecular biological function, cellular elements, and biological method (F