Illations in Bmal1 mRNA and miR-142-3p expression in the

Illations in Bmal1 mRNA and miR-142-3p expression in the mouse SCN is noteworthy; the rising phase and peak of the Bmal1 rhythm occurred in advance of peak miR-142-3p expression in the SCN, raising the possibility that Bmal1 may play a role in the order Nobiletin activation of miR-142-3p transcription. Studies using immortalized mPer2Luc SCN cells were subsequently conducted to determine whether levels of mature miR142-3p are also rhythmically regulated in vitro because this cell line has been shown to retain the endogenous oscillatory properties of the SCN in vivo (ensemble 117793 custom synthesis rhythms of Farnell et al. [29]). Similar to the temporal profile observed in the mouse SCN, miR-142-3p expression (normalized to U6 snRNA) oscillated with a circadianperiodicity (p,0.01) in cultures of mPer2Luc SCN cells 24195657 (Fig. 2A). The amplitude of this circadian rhythm in miR-142-3p levels was robust, with 3- to 4-fold differences between peak and trough values. 16574785 The rhythmic peak of miR-142-3p expression in mPer2Luc SCN cells at 12 h was significantly greater (p,0.01) than the minima observed at 20 h. Circadian variation in Bmal1 expression was also observed in the same mPer2Luc SCN cultures (p,0.01), with circadian peaks in Bmal1 mRNA levels at 0 h and 24 h that were significantly greater (p,0.01) than the nadir observed at 8 h. The Bmal1 and miR-142-3p rhythms in mPer2Luc SCN cells were marked by overt phase differences such that the oscillation in Bmal1 mRNA levels was antiphasic to the circadian profile in miR142-3p expression (Fig. 2A). This phase relationship between Bmal1 mRNA and miR-142-3p rhythms in SCN cells in vitro differed from that observed in the SCN in vivo. Although the basis for the phase differences between in vivo and in vitro SCN rhythms is unknown, an important consideration is that analysis of the mouse SCN was conducted following stable entrainment to a strong synchronizing signal (i.e., LD cycle) whereas experiments with mPer2Luc SCN cultures were performed in the absence of advance exposure to a defined phase-resetting stimulus (e.g., serum shock). To verify that the observed miR-142-3p rhythm in SCN cells is under control of the circadian clock, the temporal profile of miR142-3p expression was analyzed in a SCN cell line derived from mice with targeted disruption of Per1 and Per2 genes (Per1ldc/ Per2ldc). These mutant mice have been shown to exhibit an arrhythmic behavioral phenotype [38] and our previous findings indicate that SCN-specific circadian pacemaking function is similarly abolished in immortalized Per1ldc/Per2ldc SCN cells [29]. Bmal1 mRNA levels in Per1ldc/Per2ldc SCN cultures fluctuated over time but this variation was not characterized by circadian or other rhythmic harmonics. MiR-142-3p expression in the same cultures remained largely at constant levels and showed no significantFigure 1. Temporal patterns of miR-142-3p and Bmal1 expression in the mouse SCN. Symbols denote real-time PCR determinations (mean 6 SEM) of miR-142-3p (red) and Bmal1 mRNA (black) levels at 4-hour intervals in the SCN (n = 4-5) of mice exposed to constant darkness (DD). The plotted values correspond to the ratios of miR-142-3p signal normalized to U6 snRNA levels and of Bmal1/Ppia mRNA signal in which the maximal value for each gene was set at 100 . Asterisks indicate time points during which peak levels were significantly greater (p,0.05) than those observed during preceding or succeeding minima. doi:10.1371/journal.pone.0065300.gmiR-142-3p Modulation of BMAL1 in.Illations in Bmal1 mRNA and miR-142-3p expression in the mouse SCN is noteworthy; the rising phase and peak of the Bmal1 rhythm occurred in advance of peak miR-142-3p expression in the SCN, raising the possibility that Bmal1 may play a role in the activation of miR-142-3p transcription. Studies using immortalized mPer2Luc SCN cells were subsequently conducted to determine whether levels of mature miR142-3p are also rhythmically regulated in vitro because this cell line has been shown to retain the endogenous oscillatory properties of the SCN in vivo (ensemble rhythms of Farnell et al. [29]). Similar to the temporal profile observed in the mouse SCN, miR-142-3p expression (normalized to U6 snRNA) oscillated with a circadianperiodicity (p,0.01) in cultures of mPer2Luc SCN cells 24195657 (Fig. 2A). The amplitude of this circadian rhythm in miR-142-3p levels was robust, with 3- to 4-fold differences between peak and trough values. 16574785 The rhythmic peak of miR-142-3p expression in mPer2Luc SCN cells at 12 h was significantly greater (p,0.01) than the minima observed at 20 h. Circadian variation in Bmal1 expression was also observed in the same mPer2Luc SCN cultures (p,0.01), with circadian peaks in Bmal1 mRNA levels at 0 h and 24 h that were significantly greater (p,0.01) than the nadir observed at 8 h. The Bmal1 and miR-142-3p rhythms in mPer2Luc SCN cells were marked by overt phase differences such that the oscillation in Bmal1 mRNA levels was antiphasic to the circadian profile in miR142-3p expression (Fig. 2A). This phase relationship between Bmal1 mRNA and miR-142-3p rhythms in SCN cells in vitro differed from that observed in the SCN in vivo. Although the basis for the phase differences between in vivo and in vitro SCN rhythms is unknown, an important consideration is that analysis of the mouse SCN was conducted following stable entrainment to a strong synchronizing signal (i.e., LD cycle) whereas experiments with mPer2Luc SCN cultures were performed in the absence of advance exposure to a defined phase-resetting stimulus (e.g., serum shock). To verify that the observed miR-142-3p rhythm in SCN cells is under control of the circadian clock, the temporal profile of miR142-3p expression was analyzed in a SCN cell line derived from mice with targeted disruption of Per1 and Per2 genes (Per1ldc/ Per2ldc). These mutant mice have been shown to exhibit an arrhythmic behavioral phenotype [38] and our previous findings indicate that SCN-specific circadian pacemaking function is similarly abolished in immortalized Per1ldc/Per2ldc SCN cells [29]. Bmal1 mRNA levels in Per1ldc/Per2ldc SCN cultures fluctuated over time but this variation was not characterized by circadian or other rhythmic harmonics. MiR-142-3p expression in the same cultures remained largely at constant levels and showed no significantFigure 1. Temporal patterns of miR-142-3p and Bmal1 expression in the mouse SCN. Symbols denote real-time PCR determinations (mean 6 SEM) of miR-142-3p (red) and Bmal1 mRNA (black) levels at 4-hour intervals in the SCN (n = 4-5) of mice exposed to constant darkness (DD). The plotted values correspond to the ratios of miR-142-3p signal normalized to U6 snRNA levels and of Bmal1/Ppia mRNA signal in which the maximal value for each gene was set at 100 . Asterisks indicate time points during which peak levels were significantly greater (p,0.05) than those observed during preceding or succeeding minima. doi:10.1371/journal.pone.0065300.gmiR-142-3p Modulation of BMAL1 in.